Journal article
Dominik Michael Frei, E. Hodneland, I. Ríos-Mondragón, Anne Burtey, B. Neumann, Jutta Bulkescher, J. Schölermann, R. Pepperkok, H. Gerdes, T. Kögel
Scientific Reports, 2015
Scientific Reports, 2015
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APA
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Frei, D. M., Hodneland, E., Ríos-Mondragón, I., Burtey, A., Neumann, B., Bulkescher, J., … Kögel, T. (2015). Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer. Scientific Reports.
Chicago/Turabian
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Frei, Dominik Michael, E. Hodneland, I. Ríos-Mondragón, Anne Burtey, B. Neumann, Jutta Bulkescher, J. Schölermann, R. Pepperkok, H. Gerdes, and T. Kögel. “Novel Microscopy-Based Screening Method Reveals Regulators of Contact-Dependent Intercellular Transfer.” Scientific Reports (2015).
MLA
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Frei, Dominik Michael, et al. “Novel Microscopy-Based Screening Method Reveals Regulators of Contact-Dependent Intercellular Transfer.” Scientific Reports, 2015.
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@article{dominik2015a,
title = {Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer},
year = {2015},
journal = {Scientific Reports},
author = {Frei, Dominik Michael and Hodneland, E. and Ríos-Mondragón, I. and Burtey, Anne and Neumann, B. and Bulkescher, Jutta and Schölermann, J. and Pepperkok, R. and Gerdes, H. and Kögel, T.}
}
Abstract
Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions and bacteria can also utilize this mechanism to spread to adjacent cells and potentially evade immune detection. However, little is known about the molecular mechanism underlying this intercellular transfer process. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators. Our data indicates that cellular protrusions and tubular recycling endosomes are important for codeIT. We automated image acquisition and analysis to facilitate large-scale chemical and genetic screening efforts to identify key regulators of codeIT.